|
KMID : 0603520020070020101
|
|
Journal of Korean Association of Cancer Prevention
2002 Volume.7 No. 2 p.101 ~ p.107
|
|
|
Involvement of High Mobility Group Protein I(Y) in Basic Fibroblast Growth Factor-induced Angiogenesis
|
|
Koo Sun-Young
Kim Myoung-Sook
Baek Jin-Hyen
Moon Eun-Jeung
Bae Moon-Kyoung
Kim Kyu-Won
|
|
|
Abstract
|
|
|
|
Expression of High mobility group protein (HMG)I(Y), architectural transcription factor is elevated in a lot of cancer and highly proliferating cells such as fibroblast and smooth muscle cells. HMGI(Y) has been implicated in the control of cell proliferation and gene expression. Here we observed the expression of HMGI(Y) in proliferating Human Umbilical Vein Endothelial CeIls (HUVEC). When basic Fib개blast Growth Factor (bFGF), a well-known angiogenic factor, induced cell proliferation, HMG I(Y) mRNA was increased by RT-PCR. To investigate role of HMGI(Y) in cell proliferation, we used distamycin A, natural antibiotics which is also known as competitor of HMGI(Y). In MTT assay, bFGF induced proliferation of HUVECs but distamycin A with bFGF decreased cell proliferation. Moreover, distamycin A blocked bFGF-induced angiogenesis in vivo mouse matrigel plug assay and chorioallantoic membrane (CAM) assay. Taken together, we suggest that HMGI(Y) plays an important role in bFGF-induced angiogenesis especially through controlling of cell proliferation.
|
|
|
KEYWORD
|
|
|
High mobility group protein I(Y), basic Fibroblast Growth Factor, Angiogenesis, Proliferation, Distamycin A
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|